-Easy to use;

-No alcohol liquid is required for freezing;

-The subsequent use cost is lower;

-Better cell viability;

-The experiment has good repeatability.

Instructions:

1. Put the cryopreservation tube containing the cells into the program cooling box stored at room temperature, and cover the cooling box lid;

2. Put the program cooling box into the -80℃ refrigerator and freeze for more than 4 hours to achieve the freezing effect;

3. Take out the program cooling box, quickly move the cryotube to a liquid nitrogen environment for storage or perform other follow-up operations;

4. After use, put the program cooling box at room temperature, and wait for the program cooling box and alloy core to return to room temperature for later use.

Precautions:

1. Before putting it in the refrigerator, please make sure that the metal ring is lying flat on the bottom of the box and the lid is covered;

2. After putting it in the refrigerator, avoid close contact with other substances, and make sure that there is a 2.5cm gap around;

3. Please put it in a dry and cool place when not in use, try to avoid high temperature above 60℃ and ultraviolet light;

4. It is strongly recommended to stop the cultivation of the original cell line after checking the cell viability after freezing.